豬源H1N1/2009流感病毒自2009年爆發(fā)以來(lái)，在世界范圍迅速傳播，給人類(lèi)健康造成了嚴(yán)重威脅。重排是流感病毒進(jìn)化的主要方式之一，歷*多次流感的流行都是病毒重排的結(jié)果。

在這篇文章中，研究人員發(fā)現(xiàn)禽流感H9N2病毒與H1N1流感病毒當(dāng)同時(shí)感染同一個(gè)宿主（如豬或人）的時(shí)候，很容易發(fā)生重排，且部分重排病毒的毒力明顯提高。

       研究人員利用反向遺傳操作技術(shù)和小鼠模型對(duì)H1N1病毒和禽H9N2病毒的重排性開(kāi)展了研究，他們獲得了127種雜交病毒，從中探討雜交病毒的致病性。結(jié)果發(fā)現(xiàn)，這兩種病毒極易發(fā)生重排，重排病毒容易感染小鼠且部分重排病毒較兩株親本病毒致病力明顯提高：一半以上的雜交病毒立即感染了小鼠并且復(fù)制效率和親本毒株類(lèi)似，其中8種雜交病毒被證明比兩種親本毒株的毒力更強(qiáng)。通過(guò)對(duì)強(qiáng)毒力重排病毒基因組分析，研究人員發(fā)現(xiàn)所有強(qiáng)毒力病毒的PA基因均來(lái)自于H1N1流感病毒。

       來(lái)自世界衛(wèi)生組織的專(zhuān)家提出，在兩年前感染人們的H1N1大流行流感如今處于“后大流行”階段，以季節(jié)流感的形式流行。但是這些發(fā)現(xiàn)提示，如果發(fā)生遺傳重配，這種病毒仍然可能對(duì)公共衛(wèi)生帶來(lái)重大的威脅，因此研究人員建議在流感監(jiān)控中應(yīng)重視新型重排病毒的產(chǎn)生，尤其是那些攜帶有H1N1源PA基因的重排病毒。

原文摘要：

High genetic compatibility and increased pathogenicity of reassortants derived from avian H9N2 and pandemic H1N1/2009 influenza viruses

H9N2 influenza viruses have been circulating worldwide in multiple avian species and repeatedly infecting mammals, including pigs and humans, posing a significant threat to public health. The coexistence of H9N2 and pandemic influenza H1N1/2009 viruses in pigs and humans provides an opportunity for these viruses to reassort. To evaluate the potential public risk of the reassortant viruses derived from these viruses, we used reverse genetics to generate 127 H9 reassortants derived from an avian H9N2 and a pandemic H1N1 virus, and evaluated their compatibility, replication ability, and virulence in mice. These hybrid viruses showed high genetic compatibility and more than half replicated to a high titer in vitro. In vivo studies of 73 of 127 reassortants revealed that all viruses were able to infect mice without prior adaptation and 8 reassortants exhibited higher pathogenicity than both parental viruses. All reassortants with higher virulence than parental viruses contained the PA gene from the 2009 pandemic virus, revealing the important role of the PA gene from the H1N1/2009 virus in generating a reassortant virus with high public health risk. Analyses of the polymerase activity of the 16 ribonucleoprotein combinations in vitro suggested that the PA of H1N1/2009 origin also enhanced polymerase activity. Our results indicate that some avian H9-pandemic reassortants could emerge with a potentially higher threat for humans and also highlight the importance of monitoring the H9-pandemic reassortant viruses that may arise, especially those that possess the PA gene of H1N1/2009 origin.